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IN VITRO MICROPROPAGATION OF BACOPA MONNIERI L. USING NODAL EXPLANTS

 

Vannewada Indhu & Dr. Shruti Awasthi*
indhusohit@gmail.com & shruti.awasthi@gcu.edu.in,
Department of Life Sciences, School of Sciences, Garden City University, Bangalore, Karnataka, India

ABSTRACT

The present study investigated the impact of carbon source and light quality on the regeneration efficiency of nodal explants from Bacopa monnieri L. (Brahmi), a medicinal plant known for its therapeutic properties. Carbon sources, such as sucrose and glucose, are essential for providing energy and supporting metabolic processes in plant tissue cultures, which are crucial for the successful regeneration of shoots and roots. The study examined different carbon sources to determine their effect on the growth of nodal explants, aiming to identify the most effective one for enhancing regeneration efficiency. Similarly, light quality, which refers to the spectrum of light provided to the cultures, can significantly influence physiological processes such as photosynthesis, cell division, and photomorphogenesis.

Various light conditions, including different wavelengths and types of light (e.g., fluorescent or LED), were tested to evaluate their role in optimizing regeneration. The regeneration efficiency, which refers to the ability of the nodal explants to produce viable shoots or plantlets under these different treatments, was assessed based on parameters like shoot length, number of shoots, and overall plantlet health. While differences in regeneration efficiency were observed across various treatments, the findings are still considered preliminary. These results suggest that both carbon source and light quality influence the growth and development of Bacopa monnieri in vitro, but further experimentation involving sub-culturing and extended observation periods is necessary to draw definitive conclusions. Only with more detailed and sustained trials can the optimal conditions for enhancing regeneration efficiency be determined, which is essential for large-scale micropropagation of this important medicinal plant.

Keyword: Carbon source, regeneration, photosynthesis, preliminary, moisture content

Citation:

 

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VLEARNY Journal of Biological Sciences
1 (1) 2024, 31-37, https://vlearny.com/vjbs/
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