Shruti Awasthi1, Sheeja M.S.2, Chethana V C3, Lekshmi Shaji4
Department of Life Science, Garden City University, Bengaluru 560093,
India; Email: shruti.awasthi@gardencity.university
ABSTRACT
Silver has long been recognized as a safe and effective inorganic antibacterial and antifungal agent with wide-ranging biomedical applications. The advent of nanotechnology has further expanded its utility, with silver nanoparticles (AgNPs) offering advantages such as cost-effectiveness, stability, and accessibility of raw materials. While conventional physical and chemical methods—including solution reduction, thermal decomposition, microwave-assisted, and photochemical approaches—have been widely employed, they often involve toxic reagents and energy-intensive processes. In contrast, green synthesis using plant extracts, enzymes, or microorganisms has emerged as an eco-friendly and sustainable alternative.
In the present study, a one-pot green synthesis of AgNPs was achieved using Ocimum sanctum leaf extract under direct sunlight exposure. This approach provided a simple, environmentally benign method with advantages over traditional techniques. The synthesized nanoparticles were characterized using spectroscopic and microscopic techniques. UV–Vis spectroscopy revealed absorbance peaks between 240 and 290 nm and fluctuations in the 330–360 nm range, confirming the formation of nanoparticles. Scanning Electron Microscopy (SEM) demonstrated irregular spherical morphology with agglomerated structures. Fourier Transform Infrared Spectroscopy (FTIR) analysis indicated functional groups associated with biomolecules, including O–H (3415.90 cm⁻¹), C=O (amide I band, 1619.96–1627.95 cm⁻¹), aliphatic CH/CH₂ bending (1384.15 cm⁻¹), C–N stretching (1241.45 cm⁻¹), and C–O–C vibrations corresponding to polysaccharides and nucleic acids (1147.02 cm⁻¹). X-ray diffraction (XRD) confirmed the crystalline nature of the nanoparticles with distinctive peaks at 2θ = 18.0°. Overall, this study demonstrates an innovative, cost-effective, and sustainable green route for the synthesis of AgNPs from Tulsi leaf extract. The method yields stable nanoparticles with uniform size distribution, highlighting their potential for future biomedical and pharmaceutical applications.
KEYWORDS
Nanoparticles
Citation:Awasthi, S., Sheeja, M. S., Chethana, V. C., & Shaji, L. (2025). FORMULATION OF HERBAL ANTI-DANDRUFF SHAMPOO INCORPORATING GREEN SYNTHESIZED SILVER NANOPARTICLES FROM TULSI OCIMUM SANCTUM LEAF EXTRACT. VLEARNY Journal of Biological Sciences, 1(2), 10–17. https://doi.org/10.5281/zenodo.17210341
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